Viral ToxGlo™: A New Assay for Monitoring Viral-Induced Cytopathic Effect

Submitted on 18-12-2013

Karthik Narasimhan

Promega Pte Ltd

Viral agents are responsible for periodic epidemics (or pandemics) that result in significant morbidity and mortality often causing colossal economic impact. Although viral outbreaks can be limited/controlled by vaccination, education efforts and hygiene, there remains a need for ancillary treatment modalities. The current armamentarium of antiviral drugs is typically efficacious, but practically prone to diminished utility due to widespread use leading to viral recombination and resistance. Therefore, new methods of drug discovery are sought to identify novel antiviral compounds from previously unmined libraries. Because many medically important viruses utilize the lytic life cycle, drug discovery assays that measure host cell health can inversely report viral activity. For instance, the tetrazolium family of compounds (WST-1, XTT, MTT, and MTS) have been used to define viral infectivity by measuring changes in monolayer integrity. However, these assays are poorly suited for high throughput screening (HTS) due to poor sensitivity and/or multiple assay steps. We have developed a bioluminescent assay which measures cellular ATP. Because ATP is tightly regulated in cells, it serves as an excellent surrogate for host cell viability. Here we use four clinically significant viruses and four host cell lines to show that Viral ToxGlo™ can be utilized for determining the relative viral-burden in a sample for screening and characterizing antiviral compound potency during drug discovery.

*Note : This is the sample eposter and we follow this format for publishing our future eposter.

About Aquilus International

Aquilus International promotes, support and facilitate the development of scientific research to understand and harness the potentiality of innovations. 

Know More
Latest Updates
  • Microbiological Bio-reduction of Prochiral Carbonyl Compounds
  • Microbiology and Water Treatment Technology
  • Effect of Aminoglycosides Microbial Pathogenesis
  • Molecular Microbiology Techniques
  • Novel Approach in Food Microbiology
  • Pathology of Infectious Disease
  • Antimicrobial resistance
  • Microbial translocation
  • Infectious diseases
  • Nervous System
  • Bioinformatics
  • Infectious Complications
  • Bacterial Diseases
  • Gastrointestinal diseases
  • Surgical Infectious Disease
  • Infectious disease surveillance
  • Pelvic Inflammatory Disease
  • Fungal Diseases
  • Bacterial diagnostics
  • salmonella Infection
  • Lyme disease
  • Diagnostic Lung Specimens
  • Bartonella Species
Know More
Contact Us
  • Plot No. 754, DBK Heights
    4th Floor Ayyappa society
    Madhapur, Hyderabad - 81,  
    Telangana, India - 500081.

  • Phone Number - +918019996909, +91 40 6999 6909

    +91 40 4646 4575

  • Fax Number - +91 40 6999 6909