Microbial Burden of Diabetic Foot Ulcer in Calabar, NigeriaName : Dr. OFONIME OGBA
Affliation : Senior Lecturer
University : University Of Calabar
Country : Nigeria
Background: Foot ulcers in diabetes mellitus subjects are a leading cause of morbidity and mortality which culminates in non-traumatic amputations worldwide. Knowledge of the microbial burden in the ulcers may improve patients care and management. This prospective study was designed to isolate, identify, and carry out antibiotic susceptibility testing on bcterial isolates associated with diabetic foot ulcers among subjects in University of Calabar Teaching Hospital.
Methods: Subjects with diabetic foot ulcer were recruited after obtaining ethical clearance from the Research Committee and informed consent from the subjects. Samples were obtained from subjects using sterile swabs and subjected to microscopy and culture. Isolates were identified using standard bacteriological techniques. Antibiotic susceptibility testing was done by Kirby-Bauer method.
Results: Out of the 50 subjects recruited for the study, 31(62.0%) were females while 19(38.1%) were males with a mean age of 55.4±10.1 and a minimum age of 40.0 years. All the subjects had grade 4 wounds and Candida infections. The study recorded 100% infection rates among subjects with 70.0% polymicrobial infections. A total of 97 isolates were encountered among the 50 subjects accounting for the average of 1.94 isolates per subject. The most prevalent isolate was Staphylococcus aureus 32(32.9%), while the least prevalent pathogen was Klebsiella pneumonia 10(20.4%). Females harbored more isolates 61(62.9%) than males 36(37.1%) but there was no statistically significant effect of gender on infection rates (χ2 = 15.0, p ≥ 0.05). Erythromycin was the most effective (65.6%) against S. aureus while gram-negative bacteria were more susceptible to Augmentin (87.5%) and Ciprofloxacin(75.0%). Conclusion: The study has shown a high index of wound contamination with bacteria and fungi. The multiple antibiotic resistance of the bacterial isolates calls for the need to monitor resistance. Antifungal agents should be administered alongside antibiotics since all subjects had Candida infection.
Dr. OFONIME OGBA Currently Working As Senior Lecturer At The Department Of Medical Microbiology, In University Of Calabar, Nigeria. He Studied MSc In Calabar University And Obtained Her PhD Also From Calabar University From Nigeria. Dr. Ofonime Ogba Also Held Other Administrative Responsibilities Like; Member Of The 2016 Appointment And Promotion Committee.